In silico Multi Subunit Vaccine Design Referring Spike Glycoprotein of SARS-COV-2 (COVID-19): The World Pandemic

Contagious human coronavirus belong to family Coronaviridae and infects respiratory system causing the disease known as COVID-19 (WHO). To eradicate severe acute syndrome 2 pandemic, an effective vaccine should be developed. In current study immunoinformatics procedures were employed introduce a novel multi-epitope subunit vaccine. This can activate equally class I II leukocyte antigen antibody mediated immune responses. Spike glycoprotein (PDB Id: 6VSB) of was selected analysed using epitope database server for prediction potential immunogenic B T-cell epitopes. Population conservation studies protein predicted epitopes also performed population conservancy analysis tool resource. The two dimensional three structure authenticated PROCheck Raptor-X servers. Docking results peptide with alleles efficient binding resulted docked models stable during simulation. silico evaluation C-ImmSim showed that could concurrently elicit cell-mediated humoral Immune simulation significantly anticipated high levels IgM IgG, T-helper, T-cytotoxic cells, INF-γ.